In the vast majority of cases, the clinical condition of the couple does not allow proceeding with insemination and therefore assisted fertilization with level II and III techniques must be planned. Recognizing the financial aspect of ivf treatment, we take pride in offering cost-effective IVF in Lahore without compromising the quality of care.
There is an indication for more complex assisted fertilization techniques in the following cases:
-endocrine-ovulatory dysfunctions
-reduced ovarian reserve
-damaged tubes
-extreme seminals with problematic motility and morphology parameters
– previous failures of level I assisted fertilization techniques
– previous multiple abortions
In vitro fertilization techniques can also be programmed on a spontaneous cycle or after controlled ovarian stimulation; the choice depends on a series of factors that emerge from the woman’s clinical picture and is made with the aim of achieving a pregnancy while respecting and protecting the health of the two partners of the couple. The procedures summarized below fall within the II and III level assisted fertilization techniques.
IVF (in vitro fertilization and embryo-transfer) :
The oocytes collected from the ovarian follicles by oocyte pick-up under local anesthesia sedation are placed in contact with a portion of capacitated seminal fluid and the oocyte fertilization is expected to occur spontaneously, without any external mechanical intervention. The embryos of good biological quality that are obtained are subsequently transferred into the uterus using a thin catheter which does not cause discomfort or pain to the woman: 1-2 embryos are transferred into the uterus, at most and more rarely 3, according to legislation, while the supernumerary embryos they can be cryopreserved for any other embryo transfers.
IVF is practicable when the seminal fluid does not present particular problems and the fertilizing power of the individual spermatozoa is therefore preserved.
ICSI (intracytoplasmic sperm injection) :
The oocytes collected by pick-up are subjected to a mechanical “cleaning” operation which consists in the removal of the zoophoric cumulus that naturally surrounds the egg cells. This allows the degree of oocyte maturation to be assessed and only mature oocytes in metaphase II (MII) to be inseminated by ICSI. Every single egg is “injected” with a single, carefully selected sperm. At the Genova fertility in Pakistan, the sperm at IVF Success Rates, IVF cost and more ozoa for ICSI are chosen with natural selection using hyaluronic acid (PICSI), exactly as happens in nature on the surface of the oocyte. The embryos obtained are transferred to the uterus (2-3 maximum) and the remaining cryopreserved.
MESA, TESE (surgical sperm recovery) :
In the presence of seminal fluid with an absence of sperm due to obstructive problems, sperm recovery is attempted by taking them directly from the testicle. These are minimally invasive surgical methods that are performed under local anesthesia and sedation. The material taken is analyzed in the laboratory to search for spermatozoa to be used in icsi treatment and/or to be cryopreserved for possible future use.